English: A human oocyte is held by a glass holding pipette (left). A beveled glass pipette containing an immobilized ejaculated spermatozoon is inserted through the zona pellucida and deep into the oolemma, creating a deep furrow. Once the membrane of the oocyte is penetrated, the sperm is deposited therein.
{{Information |Description={{en|1=A human oocyte is held by a glass holding pipette (left). A beveled glass pipette containing an immobilized ejaculated spermatozoon is inserted through the zona pellucida and deep into the oolemma, creating a deep furrow.